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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which ends up in chromosome instability, and thus may perhaps add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in advertising and marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been described. Two more splice variants had been claimed but in-depth nucleotide sequence info is not still available.
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Blocking peptides are peptides that bind specifically into the focus on antibody and block antibody binding. These peptide generally has the epitope regarded through the antibody.
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This guideline outlines further more attainable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This guide outlines additional feasible optimization of cellular and immunoassay parameters to make sure the absolute best benefits are...
Antibodies sure to the blocking peptide not bind to your epitope over the target protein. By comparing the staining from the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Goal Details Cyclin E belongs towards the extremely conserved cyclin family members, whose users are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation patterns which add for the temporal coordination of each mitotic party. Cyclin E varieties a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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